Journal: Particle and Fibre Toxicology

Article Title: Stat-6 signaling pathway and not Interleukin-1 mediates multi-walled carbon nanotube-induced lung fibrosis in mice: insights from an adverse outcome pathway framework

doi: 10.1186/s12989-017-0218-0

Figure Lengend Snippet: Acute inflammation in response to pulmonary MWCNT instillation is IL-1 dependent. C57BL/6 and IL-1R1 KO mice were intratracheally administered 162 μg of MWCNTs, and samples were collected 24 h and 28 days later. a Graphical representation of the key event targeted by IL-1R1 KO. b IL-1α and IL-1β were measured in BAL fluid of WT animals. The horizontal lines indicate limits of detection; IL-1 α – below 2.0 pg and IL-1 β – 0.04 pg. c The number of total cells, mononuclear cells, and neutrophils was determined from cytospin slides generated from BAL fluid and cell concentration measurements from BAL. d The inflammatory cytokines CXCL1, IL-6, and IL-12p40 were measured in BAL fluid. e Distribution of MWCNTs was determined by hyperspectral imaging in H&E stained histology samples. Data represent mean ± SEM. n = 4–5. Statistical analysis was performed using two-way ANOVA. * p < 0.05, NS = not significant, †statistical interaction with p < 0.05, and NI = no statistical interaction

Article Snippet: Hyperspectral images were taken of H&E stained histology samples using a CytoViva nanoscale hyperspectral microscope (Cytoviva, Inc., Auburn, AL, USA).

Techniques: Generated, Concentration Assay, Imaging, Staining

Journal: Particle and Fibre Toxicology

Article Title: Stat-6 signaling pathway and not Interleukin-1 mediates multi-walled carbon nanotube-induced lung fibrosis in mice: insights from an adverse outcome pathway framework

doi: 10.1186/s12989-017-0218-0

Figure Lengend Snippet: Acute inflammation in response to pulmonary MWCNT instillation is partially STAT6 dependent. C57BL/6 and STAT6 KO mice were intratracheally administered 162 μg of MWCNTs, and samples were collected 24 h and 28 days later. a Graphical representation of the key event targeted by STAT6 KO. b IL-1α and IL-1β were measured in BAL fluid of WT animals. c The number of total cells, mononuclear cells, and neutrophils was determined from cytospin slides generated from BAL fluid and cell concentration measurements from BAL. d The inflammatory cytokines CXCL1, IL-6, and IL-12p40 were measured in BAL fluid. e Distribution of MWCNTs was determined by hyperspectral imaging in H&E stained histology samples. Data represent mean ± SEM. n = 4–5. Statistical analysis was performed using two-way ANOVA. *p < 0.05, NS = not significant, †statistical interaction with p < 0.05, and NI = no statistical interaction

Article Snippet: Hyperspectral images were taken of H&E stained histology samples using a CytoViva nanoscale hyperspectral microscope (Cytoviva, Inc., Auburn, AL, USA).

Techniques: Generated, Concentration Assay, Imaging, Staining

Journal: Pharmaceutics

Article Title: Poisonous Caterpillar-Inspired Chitosan Nanofiber Enabling Dual Photothermal and Photodynamic Tumor Ablation

doi: 10.3390/pharmaceutics11060258

Figure Lengend Snippet: ( a ) Schematic illustration and hyperspectral images of anchored/detached AuDD from the CNf backbone at pH 7.4 and 6.8. ( b ) Changes in zeta potential of CNf-Ce6-based samples at pH 7.4 and 6.8 (mean ± SD, n = 3, ** p < 0.01 compared to pH 7.4). ( c ) Ce6 emission spectra (λ ex = 400 nm, λ em = 600–750 nm) for the free Ce6 or CNf-Ce6-based samples at pH 7.4 and 6.8. ( d ) 9,10-Dimethylanthracene fluorescence change (F f –F s ) in free Ce6 or CNf-Ce6-based samples at pH 7.4 and 6.8 (lower graphs), and NIR Ce6 fluorescence (λ ex = 635 nm, λ em = 720 nm) images of free Ce6 or CNf-Ce6-based samples at pH 7.4 and 6.8 (upper images). All samples were irradiated for 10 min at a light intensity of 5.2 mW/cm 2 using a 670 nm laser source.

Article Snippet: Visualization of the CNf-based samples or AuDD at pH 7.4 or 6.8 was monitored using a Nikon microscope equipped with a visible and NIR hyperspectral camera (CytoViva, Auburn, AL, USA).

Techniques: Fluorescence, Irradiation

Journal: Pharmaceutics

Article Title: Poisonous Caterpillar-Inspired Chitosan Nanofiber Enabling Dual Photothermal and Photodynamic Tumor Ablation

doi: 10.3390/pharmaceutics11060258

Figure Lengend Snippet: ( a ) Uptake value [number (N) of internalized AuDD particles per MDA-MB-231 cell for 8 h of incubation at 37 °C] of the AuDD-based samples measured using an ICP-MS (mean ± SD, n = 3, ** p < 0.01 compared to AuDD at pH 7.4). ( b ) Flow cytometry analysis of MDA-MB-231 cells (incubation time: 8 h) treated with free Ce6, Ce6-tagged AuNP, BSA@CNf-Ce6, or AuDD/BSA@CNf-Ce6 at pH 7.4 and 6.8. ( c ) The hyperspectral images of the MDA-MB-231 cells incubated with untreated (control) or AuDD/BSA@CNf-Ce6 at pH 7.4 and 6.8.

Article Snippet: Visualization of the CNf-based samples or AuDD at pH 7.4 or 6.8 was monitored using a Nikon microscope equipped with a visible and NIR hyperspectral camera (CytoViva, Auburn, AL, USA).

Techniques: Incubation, Flow Cytometry